How nanopore sequencing works
1. Sample DNA is added to flow cell
The flow cell contains many tiny pores set into a membrane; an electric current is passed through each pore
DNA strand is unzipped
DNA base
Flow cell
membrane
2. DNA passes through nanopore
As each DNA base passes through, it disrupts the current in a unique way. These changes in the signal are recorded
3. Decoding the signal
Signal analysis identifies the order and modifications of the DNA bases. The software compares these sequences against libraries of sequences from the DNA of known brain tumours, aiding diagnosis
1. Sample DNA is added to flow cell
The flow cell contains many tiny pores set into a membrane; an electric current is passed through each pore
DNA strand is unzipped
DNA base
Flow cell
membrane
2. DNA passes through nanopore
As each DNA base passes through, it disrupts the current in a unique way. These changes in the signal are recorded
3. Decoding the signal
Signal analysis identifies the order and modifications of the DNA bases. The software compares these sequences against libraries of sequences from the DNA of known brain tumours, aiding diagnosis
1. Sample DNA is added to flow cell
The flow cell contains many tiny pores set into a membrane; an electric current is passed through each pore
DNA strand
is unzipped
Flow cell
membrane
DNA base
2. DNA passes through nanopore
As each DNA base passes through, it disrupts the current in a unique way. These changes in the signal are recorded
3. Decoding the signal
Signal analysis identifies the order and modifications of the DNA bases. The software compares these sequences against libraries of sequences from the DNA of known brain tumours, aiding diagnosis
1. Sample DNA is added to flow cell
The flow cell contains many tiny pores set into a membrane; an electric current is passed through each pore
Flow cell
membrane
DNA base
DNA strand is unzipped
2. DNA passes through nanopore
As each DNA base passes through,
it disrupts the current in a unique way. These changes in the signal
are recorded
3. Decoding the signal
Signal analysis identifies the order and modifications of the DNA bases. The software compares these sequences against libraries of sequences from the DNA of known brain tumours, aiding diagnosis
1. Sample DNA is added to flow cell
The flow cell contains many tiny pores set into a membrane; an electric current is passed through each pore
DNA strand is unzipped
DNA base
Flow cell
membrane
2. DNA passes through nanopore
As each DNA base passes through, it disrupts the current in a unique way. These changes in the signal are recorded
3. Decoding the signal
Signal analysis identifies the order and modifications of the DNA bases. The software compares these sequences against libraries of sequences from the DNA of known brain tumours, aiding diagnosis
1. Sample DNA is added to flow cell
The flow cell contains many tiny pores set into a membrane; an electric current is passed through each pore
DNA strand is unzipped
DNA base
Flow cell
membrane
2. DNA passes through nanopore
As each DNA base passes through, it disrupts the current in a unique way. These changes in the signal are recorded
3. Decoding the signal
Signal analysis identifies the order and modifications of the DNA bases. The software compares these sequences against libraries of sequences from the DNA of known brain tumours, aiding diagnosis
1. Sample DNA is added to flow cell
The flow cell contains many tiny pores set into a membrane; an electric current is passed through each pore
DNA strand
is unzipped
Flow cell
membrane
DNA base
2. DNA passes through nanopore
As each DNA base passes through, it disrupts the current in a unique way. These changes in the signal are recorded
3. Decoding the signal
Signal analysis identifies the order and modifications of the DNA bases. The software compares these sequences against libraries of sequences from the DNA of known brain tumours, aiding diagnosis
1. Sample DNA is added to flow cell
The flow cell contains many tiny pores set into a membrane; an electric current is passed through each pore
Flow cell
membrane
DNA base
DNA strand is unzipped
2. DNA passes through nanopore
As each DNA base passes through,
it disrupts the current in a unique way. These changes in the signal
are recorded
3. Decoding the signal
Signal analysis identifies the order and modifications of the DNA bases. The software compares these sequences against libraries of sequences from the DNA of known brain tumours, aiding diagnosis